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Project #10209
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#10209 : identification of sRNA in Leptospira biflexa and influence of a putative Hfq on the transcriptome
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Name of Applicant : Mathieu PICARDEAU
Date of application : 06-10-2017
Unit : Biology of Spirochetes
Location : BioTop
Phone : 8368
@ Mail : mathieu.picardeau@pasteur.fr

Project context and summary :

In a previous project (collaboration with PF2 and C3BI) , we determined the transcriptome landscape of the pathogen Leptospira interrogans (Zhukova A, Fernandes LG, Hugon P, Pappas CJ, Sismeiro O, Coppée JY, Becavin C, Malabat C, Eshghi A, Zhang JJ, Yang FX, Picardeau M. Genome-Wide Transcriptional Start Site Mapping and sRNA Identification in the Pathogen Leptospira interrogans.Front Cell Infect Microbiol. 2017 Jan 19;7:10.). In the present project, we performed RNA-seq with the PF2 on the saprophyte Leptospira biflexa and a mutant of a gene encoding a hfq-like protein. Hfq is a chaperone usually involved in the stability of sRNA in other bacteria and we would like to : 1) identify the sRNA ((intergenic regions, antisense RNA, etc) of L. biflexa wt strain 2) compare the level of expression of wt and hfq-like mutant strains.


Related team publications :
Zhukova A, Fernandes LG, Hugon P, Pappas CJ, Sismeiro O, Coppée JY, Becavin C, Malabat C, Eshghi A, Zhang JJ, Yang FX, Picardeau M. Genome-Wide Transcriptional Start Site Mapping and sRNA Identification in the Pathogen Leptospira interrogans.Front Cell Infect Microbiol. 2017 Jan 19;7:10
Service Delivery
Project Manager : christophe.becavin@pasteur.fr
Project Type : Short
Status : Closed


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