Project #10825
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#10825 : Characterization of the role of Argonaute proteins in regulating germline gene expression at the transcriptional and the post-transcriptional levels.
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Name of Applicant : Germano CECERE
Date of application : 10-01-2018
Unit : Mechanisms of epigenetic inheritance
Location : Monod - 5th floor - room 04C
Phone : 01 40 61 32 25
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Project context and summary :

This research project focuses on the characterization of the role of small RNAs and their associated Argonaute proteins in transcriptional and post-transcriptional regulation of germline gene expression. Using the nematode C. elegans, we have recently showed that one of the germline-expressed Argonaute protein, CSR-1, promotes germline transcription. However, CSR-1 also possess an endonucleolytic activity that might participate in post-transcriptional silencing. Therefore, two possible functions of the protein might regulate the germline transcriptome. 1) CSR-1 promotes specific germline transcription programs in the nucleus, and 2) negatively regulates expression of target transcripts in the cytoplasm. To gain mechanistic insights into these two functions, we aim to use RNA-seq, sRNA-seq, ChIP-seq, GRO-seq, Ribo-seq, RIP-seq, iCLIP in wild type worms, knock out and catalytic inactive mutants of CSR-1 protein at different times of germline development.

Related team publications :
Global effects of the CSR-1 RNA interference pathway on the transcriptional landscape. Cecere G, Hoersch S, O\\\'Keeffe S, Sachidanandam R, Grishok A. Nature Structural & Molecular BIology. 2014
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Project Type : Long
Status : In Progress
Publication : 10.1038/s41556-020-0462-7;10.1038/s41467-021-21691-6;10.1038/s41467-021-23615-w

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