Project #11936
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#11936 : Dissecting Peptidoglycan pathways in human near-haploid cells
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Name of Applicant : Martine Fanton d\'Andon
Date of application : 26-06-2018
Unit : Biology and Genetics of Bacterial Cell Wall
Location : Aile Bertrand b√Ętiment Duclaux RB
Phone : 0140613272
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Project context and summary :

It has been found that specific fragments of the peptidoglycan (PG), namely the Muramyl-triDAP (MTP) and Muramyl-dipeptide (MDP) are specifically recognized by the innate immune receptors Nod1 and Nod2, respectively (Girardin, 2003). However, it is still not known how these fragments reach the cytosol after being shed by bacteria. Therefore, the near-haploid human cell line HAP-1 (horizondiscovery;HAPLOGEN) has been transducted with a retroviral construct containing a promoterless GFP that will fuse with whatever gene after the construct has randomly inserted into the genome. It is possible to have several insertions in one cell. The cells will be stimulated with fluorescently labelled PG, afterwards interesting samples will be sorted by FACS sorter and the DNA will be prepared and sequenced by NGS.

Related team publications :
Girardin, 2003
Carette, 2009
Carette, 2011
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Project Type : Medium
Status : Pending

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