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Project #12049
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#12049 : Understanding the pathways of small RNA production during Meiotic Silencing by Unpaired DNA (MSUD) in the fungus Neurospora crassa
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Name of Applicant : Eugene Gladyshev
Date of application : 23-07-2018
Unit : Other
Location : Duclaux, aile Bertrand (02), RDC haut, 12A
Phone : 3993
@ Mail : eugene.gladyshev@pasteur.fr

Project context and summary :

The canonical (“textbook”) process of DNA homology search and recognition is initiated by DNA double-strand breaks and is mediated by the universally conserved recombinases of the RecA family. Using the phenomenon “Repeat Induced Point mutation” (RIP) in N. crassa as a model system, we have previously revealed the existence of another way to search for DNA homology, which does not require RecA proteins and which apparently operates on intact DNA double helices. This pathway can be extremely efficient, as it allows some fungi to detect the presence of only two gene-sized DNA repeats in the genome. Our current work on Meiotic Silencing by Unpaired DNA (MSUD) has shown that the same recombination-independent pathway may also be involved in the early steps of homologous chromosome pairing in meiosis, thus emerging as a conserved, perhaps fundamental mechanism of DNA homology search and recognition. We are now interested in further investigating this mechanism, using RIP and MSUD as two complementary recombination-independent processes. Specifically for this project, we are interested in identifying molecular genetic features that associate with (or even trigger) the production of small RNAs during MSUD in N. crassa.


Related team publications :
https://www.ncbi.nlm.nih.gov/pubmed/28721856
https://www.ncbi.nlm.nih.gov/pubmed/28459455
https://www.ncbi.nlm.nih.gov/pubmed/24699390
Service Delivery
Project Manager : blaise.li@pasteiur.fr
Project Type : Long
Status : Pending


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