Project #13174
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#13174 : Characterization of Yolk Sac Derived Progenitors in the Fetal Liver
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Name of Applicant : Laina Freyer
Date of application : 10-04-2019
Unit : Macrophages and Endothelial Cells
Location : Monod - 4th Floor - Room 15
Phone : +33 1 40 61 35 26
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Project context and summary :

Erythromyeloid progenitors (EMPs) originate from the yolk sac during early mouse development and migrate to the fetal liver via the circulation where they undergo massive expansion and differentiation into hematopoietic lineages. These events occur prior to the intraembryonic emergence of hematopoietic stem cells (HSCs). Unlike HSCs, EMPs cannot give rise to lymphoid lineages, nor can they provide long-term repopulation. As such, they are considered a transient fetal population, yet it is EMP-derived hematopoiesis that supports the growth and survival of the embryo independently of HSCs. HSC differentiation respects a hierarchy of progenitors with either lymphoid or myeloid fates and has been well documented in adult hematopoiesis. However, fetal hematopoiesis ensues from erythroid and myeloid progenitors with dual origins from either EMPs or HSCs. Using a genetic pulse chase labeling approach to distinguish these two ontogenies, we would like to explore heterogeneity on the single cell level and to build the differentiation tree among fetal liver myeloid progenitors using MARS-Seq.

Related team publications :
Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors. Gomez Perdiguero E, Klapproth K, Schulz C, Busch K, Azzoni E, Crozet L, Garner H, Trouillet C, de Bruijn MF, Geissmann F, Rodewald HR. Nature. 2015 Feb 26;518(7540):547-51. doi: 10.1038/nature13989. Epub 2014 Dec 3. PMID: 25470051
The development and maintenance of resident macrophages. Perdiguero EG, Geissmann F. Nat Immunol. 2016 Jan;17(1):2-8. doi: 10.1038/ni.3341. Review. PMID: 26681456
Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry. Iturri L, Saenz Coronilla J, Lallemand Y, Gomez Perdiguero E. J Vis Exp. 2017 Jul 17;(125). doi: 10.3791/55305. PMID: 28745620
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Project Type : Medium
Status : In Progress

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