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Project #14589
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#14589 : Development and design of new functionalities for MEMHDX, a web application dedicated to the statistical analysis and vizualization of large HDX-MS datasets.
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Organisms :
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Name of Applicant : Sebastien BRIER
Date of application : 20-11-2019
Unit : Biological NMR Technological Platform
Location : Lwoff – Ground Floor – 22RDC16
Phone : 3667
@ Mail : sebastien.brier@pasteur.fr
@ PI-Mail : inaki.guijarro@pasteur.fr

Project context and summary :

Hydrogen Deuterium eXchange followed by Mass Spectrometry (HDX-MS) is a recognized biophysical tool in structural biology capable of probing protein/ligand interactions, conformational changes, and protein folding and dynamics. Over the last decade, major improvements in the technology have been made (i.e., refrigerated UHPLC system, mass spectrometers with enhance resolution and sensitivity…) allowing the structural analysis of highly challenging biological systems. The characterization of such biological systems results in very complex HDX-MS datasets for which specific analytical software are needed. In this context, our group and the C3Bi have developed “MEMHDX” (Mixed-Effects Model for HDX experiments) to aid in the rapid statistical validation and the visualization of large HDX-MS datasets. This web application is freely accessible to the HDX-MS scientific community at the project home page http://memhdx.c3bi.pasteur.fr The current version of the application allows for the comparison of two unique conditions using only one unique charge state. This limitation has been pointed out by several MEMHDX users. The current project aims at designing and implementing new functionalities in MEMHDX to enhance its analytical capabilities. The possibilities for users to compare multiple HDX-MS conditions using multiple charge states will be introduced within the web application and the visualization tool provided by MEMHDX will be modified accordingly.


Related team publications :
Masson GR, Burke JE, Anand G, Borchers C, Brier S, Bou-Assaf G, Engen J, Faber J, Garlish R, Griffin P, Heck A, Iacob R, Jørgensen TJD, Klinman JP, Konermann L, Man P, Pascal B, Reichmann D, Skehel M, Snijder J, Strutzenberg T, Underbakke ES,Wagner C, WalesT,Walters BT,Weis D, Wilson D, Zhang Z, Zheng J,Schriemer DC, Rand KD. Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments. Nature Methods. 2019, 16(7), 595-602
Cirri E, Brier S, Assal R, Canul-Tec JC, Chamot-Rooke J, Reyes N. Consensus designs and thermal stability determinants of a human glutamate transporter. Elife. 2018 Oct 18;7. pii: e40110.
Hourdel V, Volant S, O’Brien DP, Chenal A, Chamot-Rooke J, Dillies MA, Brier S. MEMHDX: an interactive tool to expedite the statistical validation and visualization of large HDX-MS datasets. Bioinformatics. 2016; 32(22):3413-3419.
Service Delivery
Project Manager : stevenn.volant@pasteur.fr
Project Type : Short
Status : In Progress


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