Project

Hydrogen Deuterium eXchange followed by Mass Spectrometry (HDX-MS) is a recognized biophysical tool in structural biology capable of probing protein/ligand interactions, conformational changes, and protein folding and dynamics. Over the last decade, major improvements in the technology have been made (i.e., refrigerated UHPLC system, mass spectrometers with enhance resolution and sensitivity…) allowing the structural analysis of highly challenging biological systems. The characterization of such biological systems results in very complex HDX-MS datasets for which specific analytical software are needed. In this context, our group and the C3Bi have developed “MEMHDX” (Mixed-Effects Model for HDX experiments) to aid in the rapid statistical validation and the visualization of large HDX-MS datasets. This web application is freely accessible to the HDX-MS scientific community at the project home page http://memhdx.c3bi.pasteur.fr The current version of the application allows for the comparison of two unique conditions using only one unique charge state. This limitation has been pointed out by several MEMHDX users. The current project aims at designing and implementing new functionalities in MEMHDX to enhance its analytical capabilities. The possibilities for users to compare multiple HDX-MS conditions using multiple charge states will be introduced within the web application and the visualization tool provided by MEMHDX will be modified accordingly.