Project

The central part of the intercellular bridge connecting the two daughter cells during cytokinesis is a highly dense structure named the Midbody first described by Flemming in 1891. We set up an experimental protocol to purify intact, detergent-free MBRs in order to have the full proteome of this organelle. Quantitative, label-free proteomics enabled us to identify MBR proteins, that we named the “Flemmingsome”, and 489 of them were found enriched as compared to whole cell lysates, thus named \\\"Enriched Flemmingsome\\\" (Addi et al. manuscript submitted). Proteins composing the Enriched Flemmingsome were individually browsed with their Protein and Gene names for their function in cytokinesis or midbody localization on PubMed and the reference indicated on the flemmingsome website. This project aims at keeping this database up-to-date with the literature.