Project #14672
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#14672 : Flemmingsome: A Midbody Remnant Proteome Database
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Name of Applicant : Neetu Gupta-Rossi
Date of application : 10-01-2020
Unit : Membrane Traffic and Cell Division
Location : Batiment Duclaux-RDH-aile Bertrand
Phone : 00 33 (0)140 613 744
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Project context and summary :

The central part of the intercellular bridge connecting the two daughter cells during cytokinesis is a highly dense structure named the Midbody first described by Flemming in 1891. We set up an experimental protocol to purify intact, detergent-free MBRs in order to have the full proteome of this organelle. Quantitative, label-free proteomics enabled us to identify MBR proteins, that we named the “Flemmingsome”, and 489 of them were found enriched as compared to whole cell lysates, thus named \\\"Enriched Flemmingsome\\\" (Addi et al. manuscript submitted). Proteins composing the Enriched Flemmingsome were individually browsed with their Protein and Gene names for their function in cytokinesis or midbody localization on PubMed and the reference indicated on the flemmingsome website. This project aims at keeping this database up-to-date with the literature.

Related team publications :
\\\"The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX / syntenin / syndecan-4 required for completion of cytokinesis.\\\" Manuscript under revision :Addi C, Presle A, Frémont S, Cuvelier F, Rocancourt M, Milin F, Schmutz S, Chamot-Rooke J, Douché T, Duchateau M, Giai Gianetto Q, Salles A, Ménager H, Matondo M, Zimmermann P, Gupta-Rossi N/Echard A.
Rab35 GTPase Triggers Switch-like Recruitment of the Lowe Syndrome Lipid Phosphatase OCRL on Newborn Endosomes. Cauvin C, Rosendale M, Gupta-Rossi N, Rocancourt M, Larraufie P, Salomon R, Perrais D, Echard A. Curr Biol. 2016 Jan 11;26(1):120-8. doi: 10.1016/j.cub.2015.11.040. Epub 2015 Dec 24. PMID: 26725203
Engulfment of the midbody remnant after cytokinesis in mammalian cells. Crowell EF, Gaffuri AL, Gayraud-Morel B, Tajbakhsh S, Echard A. J Cell Sci. 2014 Sep 1;127(Pt 17):3840-51. doi: 10.1242/jcs.154732. Epub 2014 Jul 7. PMID: 25002399
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Status : Closed
Publication : 10.1038/s41467-020-15205-z
Global Satisfaction for this application : Excellent (5/5)

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