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Project #150
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#150 : Sequence alignment – implement algorithm in C
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Name of Applicant : Cameron MacPherson
Date of application : 15-04-2015
Unit :
Location : 65 – 2 – 9A
Phone : 0140613516
@ Mail : cameron.macpherson@pasteur.fr

Project context and summary :

The CRISPR/Cas9 technology is a recent breakthrough in rapid genetic editing. A major part of getting the technology to work is the proper design of a guide RNA that will help Cas9 target specific genomic sequences. The design of this guide RNA must take into account all possible matches along an organism’s genome with as little as 50% similarity. Such a high toleration for error means that current alignment algorithms are not well suited to the task. This issue leads to suboptimal guide RNA design and/or lengthy periods of the design process. It is a problem that is exacerbated when considering CRISPR/Cas9 for high throughput applications. The development of a new brand of sequence comparison algorithms is required.


Related team publications :
MacPherson, C. and Scherf, A. Protospacer Workbench: flexible guide-RNA design for CRISPR applications. Nature Biotechnology (In Press)
Ghorbal, M., Gorman, M., MacPherson, CR., Martins, RM., Scherf, A., and Lopez-Rubio, J-J. Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system. Nature Biotechnology. 32, 819-821 (2014).
Service Delivery
Status : Declined


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