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Project #15004
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#15004 : Parvimonas micra's impact on primary human colonic cells DNA methylation
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Organisms :
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Name of Applicant : Emma Bergsten
Date of application : 07-02-2020
Unit : Molecular Microbial Pathogenesis
Location : Duclaux - 1rst floor - room 27
Phone : 0681049691
@ Mail : emma.bergsten@pasteur.fr
@ PI-Mail : armelle.phalipon@pasteur.fr

Project context and summary :

Parvimonas micra is a gram+ anaerobic coccus bacteria associated with colorectal cancer (CRC). The stool of patients with CRC has higher Parvimonas micra concentration compared to healthy individuals. It is also present in the colonic mucosa of CRC patients. Even if we find it in the adjacent healthy tissue, it is predominantly found in the tumor. Furthermore, it is correlated with the CpG island methylator phenotype (CIMP), a particular subtype of CRC. More specifically, it is associated with the hyper-methylation of tumor suppressor gene promoters. To find out whether this bacteria is involved in the initiation of CRC, we have developed a model of a differentiated epithelial monolayer of human colonic primary cells. After co-culture with this microorganism, we found a significant increase in the global DNA methylation level of the cell genome compared to the bacteria-free control. Now we would like to determine whether this increase in DNA methylation is specific to certain regions of the genome. Therefore, we will use Illumina's Infinium MethylationEPIC BeadChip Kit.


Related team publications :
Sobhani et al., 2019. Colorectal cancer-associated microbiota contributes to oncogenic epigenetic signatures. PNAS
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Status : New


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