Project #15113
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#15113 : A cost-effective molecular Tool for Strengthening Antimalarial drug Resistance surveillance in Africa (TSARA)
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Name of Applicant : Didier Menard
Date of application : 10-03-2020
Unit : Group : Malaria Genetic and Resistance
Location : Metchnikov building 2nd floor - 2039
Phone : 9135
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Project context and summary :

In the TSARA project, we aim at developing and validating a molecular platform (Dual Index Targeted Amplicon Deep Sequencing on Illumina iSeq) and a dedicated bioinformatics pipeline for targeted high throughput sequencing of genetic loci related to antimalarial drug resistance. The iSeq Illumina platform, proposed here, is designed for dual indexing of samples, consisting of a 3’ and 5’ individual barcode, which allow the user to connect every sequence generated to a specific sample (up to 384 samples). The development and the validation of this novel molecular tool will be performed at IP Paris first by using DNA extracted from P. falciparum reference strains (3D7, Dd2 and culture-adapted Cambodian strains) (WP1) and second, by using P. falciparum DBS samples collected from two malaria endemic areas (Central African Republic and Cameroon) (WP2). In the WP3, we will host and train staff from IP Bangui and CP Cameroon to this new approach. Our final objective is that Dual Index Targeted Amplicon Deep Sequencing on iSeq along with minimal bioinformatics infrastructure operated in countries that are endemic for malaria to facilitate routine large-scale surveillance of the emergence of drug resistance and to ensure continued success of the malaria treatment policy.

Related team publications :
Ménard, D. et al. A Worldwide Map of Plasmodium falciparum K13-Propeller Polymorphisms. N Engl J Med. 2016 Jun 23;374(25):2453-64. doi: 10.1056/NEJMoa1513137.
Ménard, D. & Dondorp, A. Antimalarial Drug Resistance: A Threat to Malaria Elimination. Cold Spring Harbor Perspectives in Medicine 7, doi:10.1101/cshperspect.a025619 (2017).
Ariey F, Witkowski B, Amaratunga C, et al. A molecular marker of artemisinin-resistant Plasmodium falciparum malaria. Nature 2014; 505(7481): 50-5.
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Status : Closed
Global Satisfaction for this application : Excellent (5/5)

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