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Project #16522
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#16522 : A cost-effective molecular Tool for Strengthening Antimalarial drug Resistance surveillance in Africa (TSARA)
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Name of Applicant : Didier Menard
Date of application : 10-11-2020
Unit : Group : Malaria Genetic and Resistance
Location : Metchnikov building 2nd floor
Phone : +33 1 44 38 91 35
@ Mail : didier.menard@pasteur.fr

Project context and summary :

In the TSARA project, we aim at developing and validating a molecular platform (Dual Index Targeted Amplicon Deep Sequencing on Illumina iSeq) and a dedicated bioinformatics pipeline for targeted high throughput sequencing of genetic loci related to antimalarial drug resistance. The iSeq Illumina platform, proposed here, is designed for dual indexing of samples, consisting of a 3’ and 5’ individual barcode, which allows the user to connect every sequence generated to a specific sample (up to 384 samples). The development and the validation of this novel molecular tool will be performed at IP Paris first by using DNA extracted from P. falciparum reference strains (3D7, Dd2 and culture-adapted Cambodian strains) (WP1) and second, by using P. falciparum DBS samples collected from two malaria-endemic areas (The central African Republic and Cameroon) (WP2). In the WP3, we will host and train staff from IP Bangui and CP Cameroon to this new approach. Our final objective is that Dual Index Targeted Amplicon Deep Sequencing on iSeq along with minimal bioinformatics infrastructure operated in countries that are endemic for malaria to facilitate routine large-scale surveillance of the emergence of drug resistance and to ensure the continued success of the malaria treatment policy


Related team publications :
A molecular marker of artemisinin-resistant Plasmodium falciparum malaria. Ariey F, Witkowski B, Amaratunga C, Beghain J, Langlois AC, Khim N, Kim S, Duru V, Bouchier C, Ma L, Lim P, Leang R, Duong S, Sreng S, Suon S, Chuor CM, Bout DM, Ménard S, Rogers WO, Genton B, Fandeur T, Miotto O, Ringwald P, Le Bras J, Berry A, Barale JC, Fairhurst RM, Benoit-Vical F, Mercereau-Puijalon O, Ménard D. Nature. 2014 Jan 2;505(7481):50-5. doi: 10.1038/nature12876. Epub 2013 Dec 18. PMID: 24352242
A Worldwide Map of Plasmodium falciparum K13-Propeller Polymorphisms. Ménard D, Khim N, Beghain J, Adegnika AA, Shafiul-Alam M, Amodu O, Rahim-Awab G, Barnadas C, Berry A, Boum Y, Bustos MD, Cao J, Chen JH, Collet L, Cui L, Thakur GD, Dieye A, Djallé D, Dorkenoo MA, Eboumbou-Moukoko CE, Espino FE, Fandeur T, Ferreira-da-Cruz MF, Fola AA, Fuehrer HP, Hassan AM, Herrera S, Hongvanthong B, Houzé S, Ibrahim ML, Jahirul-Karim M, Jiang L, Kano S, Ali-Khan W, Khanthavong M, Kremsner PG, Lacerda M, Leang R, Leelawong M, Li M, Lin K, Mazarati JB, Ménard S, Morlais I, Muhindo-Mavoko H, Musset L, Na-Bangchang K, Nambozi M, Niaré K, Noedl H, Ouédraogo JB, Pillai DR, Pradines B, Quang-Phuc B, Ramharter M, Randrianarivelojosia M, Sattabongkot J, Sheikh-Omar A, Silué KD, Sirima SB, Sutherland C, Syafruddin D, Tahar R, Tang LH, Touré OA, Tshibangu-wa-Tshibangu P, Vigan-Womas I, Warsame M, Wini L, Zakeri S, Kim S, Eam R, Berne L, Khean C, Chy S, Ken M, Loch K, Canier L, Duru V, Legrand E, Barale JC, Stokes B, Straimer J, Witkowski B, Fidock DA, Rogier C, Ringwald P, Ariey F, Mercereau-Puijalon O; KARMA Consortium. N Engl J Med. 2016 Jun 23;374(25):2453-64. doi: 10.1056/NEJMoa1513137. PMID: 27332904
Emergence and clonal expansion of in vitro artemisinin-resistant Plasmodium falciparum kelch13 R561H mutant parasites in Rwanda. Uwimana A, Legrand E, Stokes BH, Ndikumana JM, Warsame M, Umulisa N, Ngamije D, Munyaneza T, Mazarati JB, Munguti K, Campagne P, Criscuolo A, Ariey F, Murindahabi M, Ringwald P, Fidock DA, Mbituyumuremyi A, Menard D. Nat Med. 2020 Oct;26(10):1602-1608. doi: 10.1038/s41591-020-1005-2. Epub 2020 Aug 3. PMID: 32747827
Service Delivery
Project Manager : amaury.vaysse@pasteur.fr
Project Type : Coaching
Status : In Progress


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