Topics : Organisms : Group : Name of Applicant :
Louis Lambrechts Date of application :
23-03-2021 Unit :
Insect-Virus Interactions Location :
26-04-07b Phone :
3497@ Mail :
Project context and summary :
Investigating the genetic basis of an organism’s phenotype typically focuses on genomic sequences annotated as genes. This traditional approach ignores repeated sequences such as transposable elements (TEs) that often make up a large fraction of the genome and contribute significantly to genetic diversity, including variation in genome size. TEs are mainly known to play an important role in genome architecture and evolution through their mutational potential but TE expression itself can also regulate gene expression and chromatin accessibility, activate cellular signaling pathways, and trigger aging or antiviral activities. In the mosquito Aedes aegypti, more than half of the genome consists of TEs but their contribution to phenotypic variation is virtually unknown. This aim of this project is to survey the variation in TE content and expression of Aedes aegypti mosquitoes using existing RNA-seq and DNA-seq datasets. It will lay the foundation for future studies on the functional relationships between TEs and phenotypes of interest.Related team publications :
Suzuki Y, Baidaliuk A, Miesen P, Frangeul L, Crist AB, Merkling SH, Fontaine A, Lequime S, Moltini-Conclois I, Blanc H, van Rij RP, Lambrechts L, Saleh MC (2020) Non-retroviral endogenous viral element limits cognate virus replication in Aedes aegypti ovaries. Current Biology 30(18): 3495-3506.
Dickson LB, Merkling SH, Gautier M, Ghozlane A, Jiolle D, Paupy C, Ayala D, Moltini-Conclois I, Fontaine A, Lambrechts L (2020) Exome-wide association study reveals largely distinct gene sets underlying specific resistance to dengue virus types 1 and 3 in Aedes aegypti. PLoS Genetics 16(5): e1008794.
Matthews BJ, Dudchenko O, Kingan SB, Koren S, Antoshechkin I, Crawford JE, Glassford WJ, Herre M, Redmond SN, Rose NH, Weedall GD, Wu Y, Batra SS, Brito-Sierra CA, Buckingham SD, Campbell CL, Chan S, Cox E, Evans BR, Fansiri T, Filipović I, Fontaine A, Gloria-Soria A, Hall R, Joardar VS, Jones AK, Kay RGG, Kodali VK, Lee J, Lycett GJ, Mitchell SN, Muehling J, Murphy MR, Omer AD, Partridge FA, Peluso P, Aiden AP, Ramasamy V, Rašić G, Roy S, Saavedra-Rodriguez K, Sharan S, Sharma A, Smith ML, Turner J, Weakley AM, Zhao Z, Akbari OS, Black WC 4th, Cao H, Darby AC, Hill CA, Johnston JS, Murphy TD, Raikhel AS, Sattelle DB, Sharakhov IV, White BJ, Zhao L, Aiden EL, Mann RS, Lambrechts L, Powell JR, Sharakhova MV, Tu Z, Robertson HM, McBride CS, Hastie AR, Korlach J, Neafsey DE, Phillippy AM, Vosshall LB (2018) Improved reference genome of Aedes aegypti informs arbovirus vector control. Nature 563(7732): 501-507.