Project #16896
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#16896 : Single-cell and spatial transcriptomics of cells of the hematopoietic and stromal lineages in the zebrafish embryo
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Name of Applicant : Anne SCHMIDT
Date of application : 14-04-2021
Unit : Macrophages and Development of Immunity
Location : Metchnikoff , floor 1
Phone : 06 81 40 86 66
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Project context and summary :

Hematopietic stem cells (HSCs) are essential for supplying blood and immune cells of the vertebrate body. They are essentially formed during the embryonic time-period, from the wall of vessels and in particular the dorsal aorta. Their survival and self-renewal capabilities are depending on environmental conditions provided by hematopoietic niches among which the bone marrow microenvironment, in adult mammals. During embryonic development, HSC precursors home in transient niches; the main organ being the fetal liver. Currently, functional characteristics of developmental hematopoietic niches - including spatially-resolved biochemical and biomechanical specificities -, are lacking. This information is mandatory not only for fundamental aspects but also for the ultimate goal of producing in vitro HSCs with regeneration potential, for the purpose of regenerative medicine. The aim of our project is to characterize HSCs and niche cells at the single cell and spatial transcriptomic levels so as to identify spatially-restricted molecular components essential for HSC survival and stemness maintenance. To do so, we will use the zebrafish embryo that offers unique advantages in comparison to other vertebrates, in particular for investigating single cell HSC-niche interactions at the level of entire hematopoietic organs.

Related team publications :
Lancino M, Majello S, Herbert S, De Chaumont F, Tinevez J-Y, Olivo-Marin J-C, Herbomel P, Schmidt A. 2018. Anisotropic organization of circumferential actomyosin characterizes hematopoietic stem cells emergence in the zebrafish. Elife 7. doi:10.7554/eLife.37355
Murayama E, Kissa K, Zapata A, Mordelet E, Briolat V, Lin H-F, Handin RI, Herbomel P. 2006. Tracing hematopoietic precursor migration to successive hematopoietic organs during zebrafish development. Immunity 25:963–975. doi:10.1016/j.immuni.2006.10.015
Murayama E, Sarris M, Redd M, Le Guyader D, Vivier C, Horsley W, Trede N, Herbomel P. 2015. NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation. Nat Commun 6:8375. doi:10.1038/ncomms9375
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Project Type : Medium
Status : In Progress

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