Project

Project #17079
Step by step one goes very far

Logged as guest


Go Back to Project List
#17079 : Comparing viral replication of intergenotypic hepatitis C virus Core recombinants
Topics :
Organisms :
Group :
Name of Applicant : Emeline Simon
Date of application : 29-06-2021
Unit : Molecular Genetics of RNA Viruses
Location : Borrel -1
Phone : +33140613124
@ Mail : emeline.simon@pasteur.fr
@ PI-Mail : sylvie.van-der-werf@pasteur.fr

Project context and summary :

Using reverse genetics, we produced recombinant viruses within a prototypic HCV strain backbone, by substituting its core coding sequence by the equivalent sequences from from clinical isolates of different HCV genotypes. To verify that heterologous core sequences did not impair cross-talk with prototypic viral elements and could be used for comparative studies, we monitored the viral replication of our set of core intergenotypic viruses. For this, we quantified the amount of intracellular viral RNA, secreted genomes and infectious titers at different time points following infection of hepatoma cells with the recombinant viruses.


Related team publications :
Service Delivery
Project Manager : elise.jacquemet@pasteur.fr
Project Type : Short
Status : In Progress


Go Back to Project List

  • Drop files here or
    Accepted file types: fasta, fa, gbk, jpg, jpeg, png, pdf, txt, ppt, pptx, doc, docx, zip, gz, ods, odt, odp, xlsx, xls, csv, r, tsv, html.