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Project #19119
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#19119 : Comparative genomics analysis of cyclic-di-GMP metabolism across the Leptospira genus
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Organisms :
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Name of Applicant : Roman Thibeaux
Date of application : 04-04-2022
Unit : Leptospirosis,Other
Location : Institut Pasteur de Nouvelle Calédonie, Médipole de Koutio
Phone : 0627381689
@ Mail : rthibeaux@pasteur.nc
@ PI-Mail : cgoarant@pasteur.nc

Project context and summary :

Finely tuned sensory systems enable bacteria to sense and respond to fluctuating environments, coordinating adaptive changes in metabolic pathways and physiological outputs. For pathogenic Leptospira, signaling pathways allow a timely expression of virulence factors during the successive steps of infection of a mammal host. As the bacteria is excreted by its host, signaling pathways enable switching the expression towards factors promoting survival in the environment. A unifying theme across bacterial species is that biofilm formation coincides with the synthesis of the cellular signaling molecule bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) and this feature seems to be conserved in Leptospira. Our current work shows that the c-di-GMP regulation pathway is a major regulatory network involved in biofilm formation, virulence and motility in the pathogen Leptospira interrogans. Biofilm production and virulence expression is quite variable across the leptospira genus (highly virulent species, low virulent species and saprophytes species showing increase biofilm production). We would like to explore how the c-di-GMP metabolism, and the many genes associated with its synthesis, and degradation have evolved across the leptospira genus. We believe that understanding the evolutionary relationship of the c-di-GMP metabolism genes in the Leptospira genus would help us to understand the contribution of this second messenger to pathogenesis and biofilm formation in the Leptospira genus


Related team publications :
The zoonotic pathogen Leptospira interrogans mitigates environmental stress through cyclic-di-GMP-controlled biofilm production. Thibeaux R, Soupé-Gilbert ME, Kainiu M, Girault D, Bierque E, Fernandes J, Bähre H, Douyère A, Eskenazi N, Vinh J, Picardeau M, Goarant C. NPJ Biofilms Microbiomes. 2020 Jun 12;6(1):24. doi: 10.1038/s41522-020-0134-1. PMID: 32532998 Free PMC article.
Revisiting the taxonomy and evolution of pathogenicity of the genus Leptospira through the prism of genomics. Vincent AT, Schiettekatte O, Goarant C, Neela VK, Bernet E, Thibeaux R, Ismail N, Mohd Khalid MKN, Amran F, Masuzawa T, Nakao R, Amara Korba A, Bourhy P, Veyrier FJ, Picardeau M. PLoS Negl Trop Dis. 2019 May 23;13(5):e0007270. doi: 10.1371/journal.pntd.0007270. eCollection 2019 May. PMID: 31120895 Free PMC article.
Deciphering the unexplored Leptospira diversity from soils uncovers genomic evolution to virulence. Thibeaux R, Iraola G, Ferrés I, Bierque E, Girault D, Soupé-Gilbert ME, Picardeau M, Goarant C. Microb Genom. 2018 Jan;4(1):e000144. doi: 10.1099/mgen.0.000144. Epub 2018 Jan 3. PMID: 29310748 Free PMC article.
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Project Manager : alexis.criscuolo@pasteur.fr
Project Type : Short
Status : In Progress


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