Project #2263
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#2263 : HIghthroughput CRISPR-Cas9 screen in bacteria
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Name of Applicant : David Bikard
Date of application : 12-07-2015
Unit : Synthetic Biology
Location : Batiment Jacob, 4ème étage
Phone : 3924
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Project context and summary :

CRISPR-Cas9 tools have recently emerged as a very powerful way to edit genomes and control gene expression. The Cas9 protein is an endonuclease that can be guided by a small guide RNA (gRNA) to any target DNA sequence of choice. This feature has enabled the construction of gRNA libraries to perform genome-wide CRISPR-Cas9 functional screens. Such screens have notably been used to identify factors involved in drug and toxin resistance in human cells. We are now in the process of developing such a screen for bacteria. In this screen, we use the catalytic null mutant of Cas9 known as dCas9 to block transcription at target positions and knockdown genes. The purpose of this project is to investigate the properties of this type of screen in bacteria.

Related team publications :
Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, and Marraffini LA (2013) Programmable repression and activation of bacterial gene expression using an engineered CRISPR-cas system. Nucleic Acids Research.
Jiang W, Bikard D, Cox D, Zhang F and Marraffini LA (2012) RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nature Biotechnology.
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Project Type : Long
Status : Closed
Publication : 10.1038/s41467-018-04209-5

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