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Project #2391
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#2391 : gRNA library design
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Name of Applicant : Pablo Navarro Gil
Date of application : 09-02-2015
Unit : Epigenetics of Stem Cells
Location : Monod (66) – 2eme etage
Phone : 0145688285
@ Mail : damien.mornico@pasteur.fr

Project context and summary :

We have recently identified around 500 long non-coding RNAs that follow a very precise expression pattern in undifferentiated mouse Embryonic Stem cells. In order to assess if any of those molecules are functionally relevant for the biology of ES cells we will perform a functional screening based on the CRISPR technology. We plan to use a modified version of the CRISPR-CAS9 technology, the CRISPRon system, in which (i) the guide-RNAs (gRNAs) will target the promoters of our candidate lncRNAs (at least 5 gRNAs per promoter) and (ii) an enzymatically-inactive CAS9 will be engineered to recrute 10 molecules of the potent VP64 transactivator. This will enable to upregulate the targeted lncRNA from its endogeneous locus.


Related team publications :
Service Delivery
Project Manager : christophe.malabat@pasteur.fr
Project Type : Medium
Status : Awaiting Publication


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