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Project #2510
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#2510 : Implementation of a statistical tool for HDX-MS data analyses
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Name of Applicant : Sébastien BRIER
Date of application : 05-11-2014
Unit : Structural Mass Spectrometry and Proteomics
Location : Centre François Jacob – 2eme étage – Office 26.02.09b
Phone : 01 44 38 93 88
@ Mail : dmornico@pasteur.fr
@ PI-Mail : julia.chamot-rooke@pasteur.fr

Project context and summary :

Hydrogen deuterium exchange detected by mass spectrometry (HDX-MS) is a powerful technique to probe the conformation and dynamics of proteins. Over the past 10 years, the HDX-MS workflow has been optimized and automatized leading to a rapid expansion of the technology in both academic lab and pharmaceutical companies. Thanks to these improvements, modern HDX-MS can be applied to investigate more complex biological systems, including large protein complexes and membrane proteins. However, the higher the size of the protein under study, the more complex the HDX-MS data. Several noncommercial and commercial software solutions have been developed to help for the analysis of HDX-MS data. We are currently using DynamX 3.0 that is a Waters-specific product specifically designed for the nanoACQUITY UPLC system with HDX technology. The aim of the project is to design and implement a statistical tool compatible with the output generated by DynamX to read ily validate results obtained with large protein complexes.


Related team publications :
Two cross-reactive monoclonal antibodies recognize overlapping epitopes on Neisseria meningitidis factor H binding protein but have different functional properties. Faleri A, Santini L, Brier S, Pansegrau W, Lo Surdo P, Scarselli M, Buricchi F, Volpini G, Genovese A, van der Veen S, Lea S, Tang CM, Savino S, Pizza M, Finco O, Norais N, Masignani V. FASEB J. 2014 Apr;28(4):1644-53. PMID: 24371123
Structural insight into the mechanism of DNA-binding attenuation of the Neisserial adhesin repressor NadR by the small natural ligand 4-hydroxyphenylacetic acid. Brier S, Fagnocchi L, Donnarumma D, Scarselli M, Rappuoli R, Nissum M, Delany I, Norais N. Biochemistry. 2012 Aug 28;51(34):6738-52. PMID: 22834735
Use of hydrogen/deuterium exchange mass spectrometry and mutagenesis as a tool to identify the binding region of inhibitors targeting the human mitotic kinesin Eg5. Brier S, Lemaire D, DeBonis S, Kozielski F, Forest E. Rapid Commun Mass Spectrom. 2006;20(3):456-62. PMID: 16402342
Service Delivery
Project Manager : stevenn.volant@pasteur.fr
Project Type : Long
Status : Closed
Publication : http://bioinformatics.oxfordjournals.org/content/early/2016/07/12/bioinformatics.btw420.long
Global Satisfaction for this application : Excellent (5/5)


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