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Project #2512
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#2512 : Single cell gene expression profiling of HIV-specific CD8+ T cells associated with HIV control
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Name of Applicant : Asier Saez-Cirion
Date of application : 02-02-2015
Unit : HIV, Inflammation and Persistence
Location : Centre François Jacob – 3eme étage – Room: 04A
Phone : 0145688944
@ Mail : asier.saez-cirion@pasteur.fr

Project context and summary :

Controlling virus replication by generating a strong CD8+ T cell response against HIV is one of the major goals in the development of an effective HIV vaccine candidate. Indeed, during the chronic phase of infection, HIV-specific CD8+ T cells were shown to have impaired functionality and fail to control viral replication. Understanding the profile of CD8+ T cells able to efficiently tackle HIV is therefore much needed. HIV controllers (HIC) are individuals who control viremia without antiretroviral therapy. CD8+ T cells seems to play a major role in their HIV control. We hypothesized that HIV-specific CD8+ T cells associated with control of infection bear particular transcriptional signature (cell cycle, survival, activation, cytolytic function…) when compared to HIV-specific CD8+ T cells not associated with control of infection sharing the same memory phenotype.


Related team publications :
Sáez-Cirión A, Lacabaratz C, Lambotte O, Versmisse P, Urrutia A, Boufassa F, Barré-Sinoussi F, Delfraissy JF, Sinet M, Pancino G, Venet A; Agence Nationale de Recherches sur le Sida EP36 HIV Controllers Study Group. Proc Natl Acad Sci U S A. 2007;104(16):6776-81
A. Sáez-Cirión, C. Bacchus, L. Hocqueloux, V. Avettand-Fenoel, I. Girault, C. Lecuroux, V. Potard, P. Versmisse, A. Melard, T. Prazuck, B. Descours, J. Guergnon, JP. Viard, F. Boufassa, O. Lambotte, C. Goujard, L. Meyer, D. Costagliola, A. Venet, G. Pancino , B. Autran and C. Rouzioux for the ANRS VISCONTI Study Group. 2013. Post-treatment HIV-1 controllers with a long-term virological remission after the interruption of early initiated antiretroviral therapy. PLoS Pathogens.
A. Sáez-Cirión, S.Y.Shin, P. Versmisse, F. Barré- Sinoussi and G. Pancino. 2010. Ex vivo T cell-based HIV suppression assay to evaluate HIV-specific CD8+ T cell responses. Nature Protocols. 5(6):1033-41
Service Delivery
Project Manager : stevenn.volant@pasteur.fr
Project Type : Long
Status : Awaiting Publication
Global Satisfaction for this application : Excellent (5/5)


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