Project #2528
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#2528 : Analysis of host epitranscriptional modifications upon colonisation with commensals and infection by bacterial pathogens
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Name of Applicant : Pascale Cossart
Date of application : 13-02-2015
Unit : Bacteria-Cell Interactions
Location : Bâtiment Roux, Rez-de-chaussée
Phone : 8841
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Project context and summary :

The colonisation of the murine gut by commensal bacteria has been shown to profoundly influence the host physiology. We would like to investigate if these effects are in part mediated by changes in epitranscriptomics, i.e. mRNA modifications influencing the stability and degradation of mRNA (Dominissini et al. Cell 2012, Meyer et al. Nature 2012). To this end, we aim to investigate the levels of the m6A modification of mRNA and differentially methylated targets in organs derived from mice with a conventional flora, germ-free mice and gnotobiotic mice colonized with a specific commensal, Akkermansia muciniphila, known for benificial effects on host metabolism (Everard et al. PNAS 2012, Shin et al. Gut 2014). Since also pathogenic bacteria have been shown to influence the host cells on many levels, including the dysregulation of transcription in the host, we would like to extend our study of host mRNA methylation patterns to mice that have been intra-gastrically infected with Listeria monocytogenes in the presence or absence of commensal bacteria.

Related team publications :
Archambaud C, Sismeiro O, Toedling J, Soubigou G, Bécavin C, Lechat P, Lebreton A, Ciaudo C, Cossart P. The intestinal microbiota interferes with the microRNA response upon oral Listeria infection. MBio. 2013 Dec 10;4(6):e00707-13.
Archambaud C, Nahori MA, Soubigou G, Bécavin C, Laval L, Lechat P, Smokvina T, Langella P, Lecuit M, Cossart P. Impact of lactobacilli on orally acquired listeriosis. Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16684-9.
Lecuit M, Sonnenburg JL, Cossart P, Gordon JI. Functional genomic studies of the intestinal response to a foodborne enteropathogen in a humanized gnotobiotic mouse model. J Biol Chem. 2007 May 18;282(20):15065-72
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Project Type : Long
Status : Closed
Publication : 10.1038/s41467-020-15126-x

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