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Project #2561
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#2561 : Identification of Transcription Start Sites and small RNAs in Leptospira interrogans by transcriptome analysis
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Name of Applicant : Mathieu Picardeau
Date of application : 03-11-2015
Unit : Biology of Spirochetes
Location : BioTop
Phone : 8368
@ Mail : mpicard@pasteur.fr

Project context and summary :

Leptospiral promoter regions are poorly characterized: experimentally proven transcription factor binding sites have not been described in the literature and promoter prediction algorithms and E. coli consensus sequences of DNA motifs are not appropriate for Leptospira. Identification of Transcription Start Sites (TSS) and promoters on a global scale will provide essential information on DNA motifs that are targets of RNA polymerases, sigma factors and transcription factors. RNA sequencing will also provide information on small regulatory RNAs. These small (~30-500 nt) non-coding RNAs (sRNAs) are an emerging class of post-transcriptional regulators which play a variety of important roles in many biological processes. Studies on sRNA regulation of gene expression in Leptospira are currently in their infancy. Our results will provide new insights into the transcriptional landscape of L. interrogans, including the repertoire of sRNA, and it will establish the foundation for future experimental work on gene regulation.    


Related team publications :
A putative regulatory genetic locus modulates virulence in the pathogen Leptospira interrogans. Eshghi A, Becam J, Lambert A, Sismeiro O, Dillies MA, Jagla B, Wunder EA Jr, Ko AI, Coppee JY, Goarant C, Picardeau M. Infect Immun. 2014 Jun;82(6):2542-52.
Genomics, proteomics, and genetics of leptospira. Picardeau M. Curr Top Microbiol Immunol. 2015;387:43-63.
Service Delivery
Project Manager : anna.zhukova@pasteur.fr
Project Type : Medium
Status : Closed
Publication : 10.3389/fcimb.2017.00010
Global Satisfaction for this application : Pretty Good (4/5)


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