Project

Project #2741
Step by step one goes very far

Logged as guest


Go Back to Project List
#2741 : A reference panel of dengue vector genomes
Topics :
Organisms :
Group :
Name of Applicant : Louis Lambrechts
Date of application : 11-11-2015
Unit : Insect-Virus Interactions
Location : 26-04-07B
Phone : 0140623497
@ Mail : louis.lambrechts@pasteur.fr

Project context and summary :

Dengue prevention relies primarily on controlling populations of the main mosquito vector, Aedes aegypti, which is failing in many parts of the world because of the lack of sustained commitment of resources and ineffective implementation. Novel entomological approaches to dengue control are being developed that aim at replacing or suppressing mosquito vector populations. Insufficient genomic resources for Ae. aegypti, however, have until now impeded progress in both basic and applied research on this medically important mosquito species. The only available reference genome for Ae. aegypti is a draft that consists of over 4,800 unassembled fragments with incomplete annotation. Moreover, the inbred Ae. aegypti laboratory strain that was sequenced does not universally represent the considerable genetic and ecological diversity of the species worldwide. The large size of the genome and its high content in repeat-rich sequences of transposable elements was a major difficulty to assemble the Ae. aegypti genome sequence. In the present project, we aim to overcome this difficulty using a novel strategy for genome sequencing and assembly. The ultimate goal is to produce several, fully assembled, well-annotated, new Ae. aegypti reference genomes from epidemiologically relevant populations. The expected outcome is a genome reference panel including a catalog of species-wide genetic variation that will significantly improve genomic resources for Ae. aegypti research and help address a broad range of biological questions related to Ae. aegypti vectorial capacity and dengue virus transmission.


Related team publications :
Fansiri T, Fontaine A, Diancourt L, Caro V, Thaisomboonsuk B, Richardson JH, Jarman RG, Ponlawat A & Lambrechts L (2013) Genetic mapping of specific interactions between Aedes aegypti mosquitoes and dengue viruses. PLoS Genet 9(8): e1003621.
Service Delivery
Project Manager : varun.khanna@pasteur.fr
Project Type : Medium
Status : Closed
Global Satisfaction for this application : Pretty Good (4/5)


Go Back to Project List