Topics : Organisms : Group : Name of Applicant :
Gerald Spaeth Date of application :
01-12-2015 Unit :
Molecular Parasitology and Signaling Location :
Calmette 2nd floor Phone :
poste 3858@ Mail :
Project context and summary :
The Sudanese L. donovani strain Ld1SA is the most important experimental reference strain in our field and has been used for various systems level analysis (DNAseq, RNAseq, proteomics). However, all these analyses are strongly compromised by the fact that the current L. donovani genome reference strain LdBPK is from Nepal and very different from the Sudanese isolate (only 60% of DNAseq reads can be mapped).Related team publications :
Yau WL, Pescher P, MacDonald A, Hem S, Zander D, Retzlaff S, Blisnick T, Rotureau B, Rosenqvist H, Wiese M, Bastin P, Clos J, Späth GF. The Leishmania donovani chaperone cyclophilin 40 is essential for intracellular infection independent of its stage-specific phosphorylation status. Mol Microbiol. 2014 Jul;93(1):80-97. doi: 10.1111/mmi.12639. Epub 2014 May 23. PubMed PMID: 24811325.
Pescher P, Blisnick T, Bastin P, Späth GF. Quantitative proteome profiling informs on phenotypic traits that adapt Leishmania donovani for axenic and intracellular proliferation. Cell Microbiol. 2011 Jul;13(7):978-91. doi: 10.1111/j.1462-5822.2011.01593.x. Epub 2011 Apr 26. PubMed PMID: 21501362.
Morales MA, Watanabe R, Dacher M, Chafey P, Osorio y Fortéa J, Scott DA, Beverley SM, Ommen G, Clos J, Hem S, Lenormand P, Rousselle JC, Namane A, Späth GF. Phosphoproteome dynamics reveal heat-shock protein complexes specific to the Leishmania donovani infectious stage. Proc Natl Acad Sci U S A. 2010 May 4;107(18):8381-6. doi: 10.1073/pnas.0914768107. Epub 2010 Apr 19. PubMed PMID: 20404152; PubMed Central PMCID: PMC2889574.