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Project #3100
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#3100 : SNP based analysis of French Bordetella pertussis isolates: comparison of isolates producing all the vaccine antigens to isolates producing only some of them.
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Name of Applicant : Valérie Bouchez
Date of application : 11-01-2016
Unit : Molecular Prevention and Therapy of Human Diseases
Location : Batiment Roux-porte 53A-rdc droite
Phone : 0145688333
@ Mail : valerie.bouchez@pasteur.fr
@ PI-Mail : benoit.garin@pasteur.fr

Project context and summary :

Whooping cough is a vaccine-preventable disease due to Bordetella pertussis. Even if vaccination has allowed the control of the disease, isolates are still circulating and cyclic increases of incidence are observed every 3 to 5 years even in vaccinated countries. Most developed countries now use acellular vaccines containing 3 to 5 vaccine antigens (pertussis toxin (PT), filamentous hemagglutinin (FHA), pertactin (PRN) fimbrial proteins (FIM2/FIM3)) that have replaced whole cell vaccines. In regions vaccinating with acellular vaccines with a high coverage, isolates no more producing some vaccine antigens (mainly PRN) have been reported in the last years.   Bordetella pertussis reference genome has been fully annotated in 2003 by the Sanger Institute. Analysis and comparison of different B.pertussis genomic sequences showed that circulating B.pertussis isolates differ from vaccine and reference strains. Genome evolution is characterized by gene deletions, antigenic divergences, SNP accumulations…Recent genomic analysis gathering isolates from different countries showed that the worldwide B. pertussis population has evolved in the last 60 years,. Gene categories under selection were identified underlying that Bvg-activated genes and genes coding for surface-exposed proteins were important for adaptation. However these analyses concerned only overall vaccine antigen producing isolates.   The PTMMH Unit includes the National Center of reference for Bordetellosis. In the last years some particular B.pertussis French isolates no more producing PRN but also FHA or PT have been collected, analyzed and sequenced. We would like to further analyze these genomic data with a focus on the vaccine antigen deficient isolates through a SNP-based comparison of these isolates vs co-circulating isolates producing all vaccine antigens and vs a reference strain.


Related team publications :
New Data on Vaccine Antigen Deficient Bordetella pertussis Isolates.Bouchez V, Hegerle N, Strati F, Njamkepo E, Guiso N.Vaccines (Basel). 2015 Sep 14;3(3):751-70. doi: 10.3390/vaccines3030751.PMID:26389958
Bordetella pertussis and pertactin-deficient clinical isolates: lessons for pertussis vaccines.Hegerle N, Guiso N.Expert Rev Vaccines. 2014 Sep;13(9):1135-46. doi: 10.1586/14760584.2014.932254. Epub 2014 Jun 23. eview.PMID:24953157
Analysis of Bordetella pertussis clinical isolates circulating in European countries during the period 1998-2012.van Gent M, Heuvelman CJ, van der Heide HG, Hallander HO, Advani A, Guiso N, Wirsing von Kőnig CH, Vestrheim DF, Dalby T, Fry NK, Pierard D, Detemmerman L, Zavadilova J, Fabianova K, Logan C, Habington A, Byrne M, Lutyńska A, Mosiej E, Pelaz C, Gröndahl-Yli-Hannuksela K, Barkoff AM, Mertsola J, Economopoulou A, He Q, Mooi FR.Eur J Clin Microbiol Infect Dis. 2015 Apr;34(4):821-30. doi: 10.1007/s10096-014-2297-2. Epub 2014 Dec 20.
Service Delivery
Project Manager : pierre.lechat@pasteur.fr
Project Type : Medium
Status : Closed


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