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Project #3127
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#3127 : Interactions and dynamics of fungal and bacterial microbiome in healthy people
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Name of Applicant : Marie-Elisabeth Bougnoux
Date of application : 12-01-2016
Unit : Fungal Biology and Pathogenicity
Location : Fernbach
Phone : 0140 61 31 26
@ Mail : bougnoux@pasteur.fr
@ PI-Mail : christophe.denfert@pasteur.fr

Project context and summary :

The gastrointestinal tract of humans is colonized by hundreds of microbial species, – bacteria, archaebacterial, fungi, protozoa and viruses -, collectively named the gut microbiome. The intestinal commensal bacteria have an important role in metabolic processes and contribute to colonization resistance against intestinal pathogens. Fungi are usually considered to be a minor component of the global microbiome. However, the mycobiome (fungal component of the entire microbiome) has been in fact little studied particularly with regards to its relationships with the other components of the microbiome. It seems however that fungi can be important players of the microbiome because some fungal species are able to proliferate in response to diet or during dysbiosis due to antibiotic treatment or gut inflammation. Consensus approaches to explore mycobiome together with other components of the mycobiome are still lacking. In this context the primary goal of our project will be to determine the best means to analyze bacterial and fungal microbiome concomitantly, using an identical technical procedure. We will evaluate the effectiveness of different methods for: 1) sample conservation; 2) DNA extraction; 3) mapping fungal and bacterial databases. The best procedure then will be used on samples from different studies to analyze interactions and respective dynamics of fungal and bacterial microbiome in different clinical settings.


Related team publications :
Candida albicans commensalism in the gastrointestinal tract. Neville BA, d’Enfert C, Bougnoux ME. FEMS Yeast Res. 2015 Nov;15(7). pii: fov081. doi: 10.1093/femsyr/fov081. Epub 2015 Sep 6.
Candida albicans is not always the preferential yeast colonizing humans: a study in Wayampi Amerindians.. Angebault C, Djossou F, AbĂ©lanet S, Permal E, Ben Soltana M, Diancourt L, Bouchier C, Woerther PL, Catzeflis F, Andremont A, d’Enfert C, Bougnoux ME. J Infect Dis. 2013 Nov 15;208(10):1705-16. doi: 10.1093/infdis/jit389. Epub 2013 Jul 31.
Service Delivery
Project Manager : amine.ghozlane@pasteur.fr
Project Type : Short
Status : Awaiting Publication
Global Satisfaction for this application : Excellent (5/5)


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