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Project #3254
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#3254 : ANALYSIS OF TRANSCRIPTIONAL MODULATIONS RELATED TO CELL DEATH PROCESSES IN MURINE BONE-MARROW DERIVED MACROPHAGES AND DENDRITIC CELLS INFECTED BY LEISHMANIA AMAZONENSIS
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Name of Applicant : Hervé Lecoeur
Date of application : 20-01-2016
Unit : Molecular Parasitology and Signaling
Location : calmette, batiment 12
Phone : 94-26
@ Mail : helecoe@pasteur.fr
@ PI-Mail : gerald.spaeth@pasteur.fr

Project context and summary :

Aim : In vitro infection of innate immune cells by L. amazonensis (L.am.) seems to be associated to an increase in resistance to cell death of infected cells. This project aims at deciphering the impact of in vitro L. amazonensis (L.am.) amastigotes infection on cell death processes including apoptosis, autophagy, pyroptosis and necroptosis in different host cells, i.e. Bone-marrow- derived macrophages (BMDMs) and dendritic cells (BMDCs) after one day of infection. Material : RNA samples were obtained from control and infected BMDMs (BALB/c mice) or BMDCs (BALB/c, C57BL/6 and DBA/2 mice) after their sorting by Fluorescence Activated- Cell sorting. For BALB/c BMDCs, amastigotes were also added to cells in presence of immune serum to trigger their opsonization. Samples were analysed a few years ago at the « Plateforme Transcriptome et Epigénome » with the Affymetrix technology. RNAs from BMDMs and BMDCs were analysed with the Affymetrix Mouse430_2 GeneChips and the Affymetrix Mouse Gene ST 1.0 arrays respectively.  


Related team publications :
Transcriptional signatures of BALB/c mouse macrophages housing multiplying Leishmania amazonensis amastigotes. Osorio y Fortéa J, BMC Genomics. 2009 Mar 20;10:119. doi: 10.1186/1471-2164-10-119.
Reprogramming neutral lipid metabolism in mouse dendritic leucocytes hosting live Leishmania amazonensis amastigotes. Lecoeur H. PLoS Negl Trop Dis. 2013 Jun 13;7(6):e2276. doi: 10.1371/journal.pntd.0002276. Print 2013.
Distinct transcriptional signatures of bone marrow-derived C57BL/6 and DBA/2 dendritic leucocytes hosting live Leishmania amazonensis amastigotes. Giraud E. PLoS Negl Trop Dis. 2012;6(12):e1980. doi: 10.1371/journal.pntd.0001980. Epub 2012 Dec 13..
Service Delivery
Project Manager : hugo.varet@pasteur.fr
Project Type : Short
Status : Closed
Global Satisfaction for this application : Excellent (5/5)


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