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Project #3826
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#3826 : Analysis of sequencing data from a Leishmania donovani cosmid library
Topics :
Organisms :
Group :
Name of Applicant : Pascale Pescher
Date of application : 15-02-2016
Unit : Molecular Parasitology and Signaling
Location : Calmette – 02 – 06c
Phone : 01 40 61 35 32
@ Mail : pascale.pescher@pasteur.fr
@ PI-Mail : gerald.spaeth@pasteur.fr

Project context and summary :

Chromosome amplification is commonly used by Leishmania during adaptation to environment.  In this context it is challenging to look for genes relevant for parasite virulence/attenuation/drug resistance… To restrict this chromosomal amplification, a cosmid approach (CoSeq) has been chosen to select for genes that provide fitness gain to  Leishmania donovani parasites in culture and in the animal. Therefore, a cosmid library has been generated with genomic DNA from the parasites which needs to be sequenced to control for genome coverage before transfection to the parasites. The transfected parasites will then be injected to animals or submitted to different culture conditions. Only those transfected with cosmids providing advantage under the studied conditions will be selected and will replicate. These cosmids will be extracted from the parasites and will be sequenced to reveal genes relevant for the parasite survival. The C3Bi would be implicated in the analyses of the sequencing data obtained from the PF1 (retrieve the data, mapping of the reads to Leishmania genome, estimation of the genome coverage, listing of genes selected for a given condition…).


Related team publications :
Quantitative proteome profiling informs on phenotypic traits that adapt Leishmania donovani for axenic and intracellular proliferation. Pescher P, Blisnick T, Bastin P, Späth GF. Cell Microbiol. 2011 Jul;13(7):978-91.
Service Delivery
Project Manager : giovanni.bussotti@pasteur.fr
Project Type : Medium
Status : In Progress


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