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Project #6803
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#6803 : Identification of promoteur-overlaping antisense transcription
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Name of Applicant : Gwenael Badis-Bréard
Date of application : 13-07-2016
Unit : Genetics of Macromolecular Interactions
Location : 68-04-4018
Phone : 33 31
@ Mail : gbreard@pasteur.fr
@ PI-Mail : jacquier@pasteur.fr

Project context and summary :

Analysing the transcriptome of exponentially grown or stationary phase yeasts in a genetic background that stabilises pervasive transcipts, we identified a first subset of ≈ 140 antisense transcripts anti-correlated with gene transcripts that are specifically expressed in quiescence. We are further investigating whether these genes are subject to a transcriptional interference and what are the mechanisms underlying this regulation. More in detail, we would like to analyse the loci where an antisense ncRNA are detected.


Related team publications :
Elife. 2015 Apr 23;4. doi: 10.7554/eLife.06722. Quality control of transcription start site selection by nonsense-mediated-mRNA decay. Malabat C, Feuerbach F, Ma L, Saveanu C, Jacquier A.
Widespread bidirectional promoters are the major source of cryptic transcripts in yeast. Neil H, Malabat C, d\’Aubenton-Carafa Y, Xu Z, Steinmetz LM, Jacquier A. Nature. 2009 Feb 19;457(7232):1038-42. doi: 10.1038/nature07747. Epub 2009 Jan 25.
Cell. 2005 Jun 3;121(5):725-37. Cryptic pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase. Wyers F1, Rougemaille M, Badis G, Rousselle JC, Dufour ME, Boulay J, Régnault B, Devaux F, Namane A, Séraphin B, Libri D, Jacquier A.
Service Delivery
Project Manager : bertrand.neron@pasteur.fr
Project Type : Short
Status : Closed
Global Satisfaction for this application : Excellent (5/5)


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