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Project #7066
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#7066 : Insect Vector Genomics
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Name of Applicant : Ken Vernick
Date of application : 08-09-2016
Unit : Genetic and Genomics of Insects Vectors
Location : B√Ętiment Sergent – 3e Etg
Phone : 0140613642
@ Mail : kvernick@pasteur.fr

Project context and summary :

Insect vectors durably transmit many important human and animal diseases. Insects are mobile, adaptable and difficult to control, which makes them efficient vehicles for disease emergence, spread and maintenance. Genomic tools have been applied to the study of vectors and vector control, and some insects such as the African malaria vector Anopheles gambiae have now become new model organisms for natural host-pathogen interactions. We study mosquito vectors of malaria and arboviruses, which generates four main kinds of large-scale data that requires dedicated bioinformatics expertise: i) functional dissection of mosquito immune signaling pathways by RNAseq transcriptome profiling to detect responses to pathogen infection and/or silencing of target genes, including mRNA as well as small and other non-coding RNAs, ii) next-gen genetic linkage mapping by deep sequencing of index-tagged phenotyped individual mosquitoes, iii) population genomic analysis by whole-genome sequencing of hundreds of individual 250Mb mosquito genomes, iv) metagenomic analysis of the mosquito microbiome and pathogen susceptibility, and of ecological metagenomic communities in field samples of mosquito vectors.


Related team publications :
Carissimo, G., E. Pondeville, M. McFarlane, I. Dietrich, C. Mitri, E. Bischoff, C. Antoniewski, C. Bourgouin, A. B. Failloux, A. Kohl and K. D. Vernick (2015). “Antiviral immunity of Anopheles gambiae is highly compartmentalized, with distinct roles for RNA interference and gut microbiota.” Proc Natl Acad Sci U S A 112(2): E176-185.
Redmond, S. N., K. Eiglmeier, C. Mitri, K. Markianos, W. M. Guelbeogo, A. Gneme, A. T. Isaacs, B. Coulibaly, E. Brito-Fravallo, G. Maslen, D. Mead, O. Niare, S. F. Traore, N. Sagnon, D. Kwiatkowski, M. M. Riehle and K. D. Vernick (2015). “Association mapping by pooled sequencing identifies TOLL 11 as a protective factor against Plasmodium falciparum in Anopheles gambiae.” BMC Genomics 16(1): 779.
Riehle, M. M., W. M. Guelbeogo, A. Gneme, K. Eiglmeier, I. Holm, E. Bischoff, T. Garnier, G. M. Snyder, X. Li, K. Markianos, N. Sagnon and K. D. Vernick (2011). “A cryptic subgroup of Anopheles gambiae is highly susceptible to human malaria parasites.” Science 331(6017): 596-598.
Service Delivery
Project Manager : adrien.pain@pasteur.fr
Project Type : Long
Status : In Progress
Publication : 10.7554/eLife.25813 ; 0.1186/s12864-018-4918-0


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