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Project context and summary :
After the behavioural characterisation of the Shank3 KO cohort, we extracted and dissected different region of the brain: cortex, hippocampus, striatum, cerebellum.
These regions were selected according to the expression level of Shank3 (Peça et al. 2011). Using QUIAGEN miRNAeasy with DNAse, miRNA-enriched RNA was extracted from the brain regions in order to perform RNAseq.
We will ask 3 questions:
What is the pattern of Shank3 isoforms in different brain regions?
What are the genes/pathways differentially expressed in wild-type and Shank3 knock-out mice?
What are the genes/pathways associated with the severity of the self grooming behaviour ?
Beside data driven eperiments, we will test candidate genes/pathways such as glutamatergic receptors and <Related team publications :
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