Project

Topics :
Organisms :
Group :
Name of Applicant : BOURGERON Thomas
Date of application : 13-09-2016
Unit : Human Genetics and Cognitive Functions
Location : 68-1-21
Phone : 3943
@ Mail : aferhat@pasteur.fr
@ PI-Mail : thomas.bourgeron@pasteur.fr

---

Project context and summary :

After the behavioural characterisation of the Shank3 KO cohort, we extracted and dissected different region of the brain: cortex, hippocampus, striatum, cerebellum.

These regions were selected according to the expression level of Shank3 (Peça et al. 2011). Using QUIAGEN miRNAeasy with DNAse, miRNA-enriched RNA was extracted from the brain regions in order to perform RNAseq.

We will ask 3 questions:

• What is the pattern of Shank3 isoforms in different brain regions?

• What are the genes/pathways differentially expressed in wild-type and Shank3 knock-out mice?

• What are the genes/pathways associated with the severity of the self grooming behaviour ?

Beside data driven eperiments, we will test candidate genes/pathways such as glutamatergic receptors and <

Related team publications :