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Project #7631
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#7631 : Differentiation of Shigella species from Escherichia coli by MALDI-TOF mass spectrometry
Topics :
Organisms :
Group :
Name of Applicant : Sophie Lefèvre
Date of application : 21-12-2016
Unit : Enteric Bacterial Pathogens
Location : Biotop-3rd floor-12.03.11A
Phone : 01 45 68 83 44
@ Mail : sophie.lefevre@pasteur.fr
@ PI-Mail : francois-xavier.weill@pasteur.fr

Project context and summary :

Shigella species and E. coli are very closely related bacteria belonging to the Enterobacteriaceae family. Phenotypically they are very similar and genotypically they could be considered the same species. The differentiation of Shigella species from E. coli is a significant diagnostic challenge for the clinical microbiology laboratories. They increasingly use maldi-tof mass spectrometry-based microbial identification systems but the latter are currently not able to distinguish these species. As the National Reference Center of E. coli and Shigella, we possess an exhaustive and extensive collection of strains (all serotypes and a wide range of phenotypic profils). We want to use our unique strain collection to propose a highly performant approach for differentiating these bacterial species by MALDI-TOF MS. Success of this study would be incredibly valuable for making an immediate impact on the clinical microbiology diagnosis.


Related team publications :
doi:10.1128/JCM.01526-13
http://dx.doi.org/10.1016/j.ijmm.2014.06.004
Service Delivery
Project Manager : varun.khanna@pasteur.fr
Project Type : Short
Status : In Progress


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