Project #7750
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#7750 : Gene ontology analysis of RNAseq data from uninfected and Leishmania-infected mouse macrophages
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Name of Applicant : Eric Prina
Date of application : 16-01-2017
Unit : Molecular Parasitology and Signaling
Location : Calmette (62), 2nd floor, room 5c
Phone : 3514
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Project context and summary :

Gene ontology analysis of RNAseq data from uninfected and Leishmania-infected mouse macrophages.  Scientific context During the course of cutaneous or visceral disease in humans or experimental animal models, the resolution of leishmanial infections or the control of parasite growth is dependent on appropriate innate and adaptive immune responses developed by the parasitized host. Leishmania largely evades and subverts these responses by its intracellular life style inside the mammalian host, where the parasites develop into amastigotes mainly within macrophages (BMDMs). We have focused our interest in the BMDM inflammasome and the way Leishmania amastigotes interfere or subvert BMDM inflammatory responses. Our recent data are in favor of an absence of stimulation, even a down-regulation of the inflammasome in BMDMs harboring replicating amastigotes at the gene and protein expression levels. To go further on this, we have performed RNAseq experiments on uninfected and infected BMDMs. This project was done at the “Transcriptome and Epigenome” platform and in close collaboration with the C3BI for normalization and statistical analysis procedures. Objective In the present proposal we will perform a deep analysis of the repartition of modulated genes between the different conditions using these RNAseq data. Using C3Bi expertise we will classify known functions of modulated genes into GO aspects i.e. molecular function, cellular component and biological process, visualize gene annotations and perform statistical analyses for the distribution of the annotated genes over the GO hierarchy for the different gene lists analyzed. Hopefully, these analyses will bring us a better understanding of the mechanisms underlying the subversion of BMDM functions in the innate and adaptive immune response to Leishmania infection which is a prerequisite to design novel anti-parasitic intervention strategies targeting the infected host cell rather than the parasite.

Related team publications :
Rachidi, N. et al. Antimicrob Agents Chemother. 2014. PMID: 24366737
Lecoeur, H. et al. PLoS Negl Trop Dis. 2013. PMID: 23785538
Osorio y Fortea, J. et al. BMC Genomics. 2009. PMID: 19302708
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Project Type : Short
Status : Closed

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