Project #7810
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#7810 : Quantitative temporal analysis of cellular proteins regulated by HBV in primary human hepatocytes
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Name of Applicant : Christine Neuveut
Date of application : 31-01-2017
Unit : Hepacivirus and Innate Immunity
Location : 22-3-314
Phone : 0145688776
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Project context and summary :

Despite effective prevention against HBV infection, 300 million people worldwide are chronic HBV carriers, of whom 25% will die of liver cirrhosis or hepatocellular carcinoma (HCC). Current treatments for chronic hepatitis B (CHB) are inefficient to completely clear the virus and liver cancer is a lethal disease, thus representing an area of highly unmet medical need. Viral persistence is due to the maintenance, in the nuclei of infected cells, of the viral nuclear DNA : the cccDNA that is not targeted by the antiviral treatment and to the impairment of both the innate and adaptive immune responses that accompanies CHB infection. Viral replication depends on a balance between factors that benefit and those that restrict viral infection. However little is known about cellular factors that repress HBV replication. Using quantitative temporal viromics approach developed by P. Lenher in Cambridge we have performed a quantitative analysis of temporal changes in host and viral proteins in primary human hepatocytes through the course of HBV infection. We will in particular search for cellular factors involved in virus restriction and uncover the mechanism of viral escape.

Related team publications :
Rivière L., Gerossier L., Ducroux A., Dion S., Deng Q., Michel M.L. , Buendia M.A., Hantz O. and Neuveut C. (2015). HBx relieves chromatin-mediated transcriptional repression of hepatitis B viral cccDNA involving SETDB1 histone methyltransferase. J. Hepatol. doi: 10.1016/j.jhep.2015.06.023
Ducroux A, Benhenda S, Rivière L, Semmes OJ, Benkirane M, Neuveut C. (2014). The Tudor domain protein Spindlin1 is involved in intrinsic antiviral defense against incoming hepatitis B Virus and herpes simplex virus type 1. PloS Pathog. 10 : e1004343.
Benhenda S., Ducroux A.,Rivière L., Sobhian B., Ward M., Dion S., Hantz O., Protzer U., Michel M.L., Benkirane M., Semmes O.J., Marie-Annick Buendia M.A. and Neuveut C. (2013). The PRMT1 methyltransferase is a binding partner of HBx and a negative regulator of hepatitis B virus transcription. J. Virol. : 87
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Project Type : Short
Status : Declined

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