Project #8001
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#8001 : Characterization of an RNA binding protein of Listeria monocytogenes
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Name of Applicant : Alessandro Pagliuso
Date of application : 04-03-2017
Unit : Bacteria-Cell Interactions
Location : Roux, rdc, 16
Phone : 0782192527
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Project context and summary :

We are analyzing a Listeria protein secreted in the supernatant. From cells transfected with this protein, after crosslinking, we have isolated RNA and found the protein in the RNA fraction. In addition, the protein binds several splicing factors in RNA dependent manner. Furthemore, the protein localizes to the nucleus. In this project, we have isolated the protein from the supernatant and from bacterial extract after growth in BHI. We have extracted the RNA from the supernatant fraction containing the protein and from the bacterial extract containing the protein. We want to alalyze the RNA in these different fractions.

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Project Type : Short
Status : Closed
Publication : 10.1016/j.chom.2019.10.004

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