Project #8328
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#8328 : Experimental evolution of Chikungunya and Zika viruses’ strains to study emerging variants.
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Name of Applicant : Marco Vignuzzi
Date of application : 19-05-2017
Unit : Viral Populations and Pathogenesis
Location : Francois jacob, 5th floor
Phone : 3486
@ Mail :

Project context and summary :

Chikungunya and Zika viruses have recently extended to previously disease free areas. In addition, their high mutations rates and fast replication lead to the emergence of new strains potentially able to disseminate in these new territories, through the apparition of novel characteristics. The African (MR-766) and American (PRVABC-59) Zika strains, and the La Reunion Island and Caribbean Chikungunya strains, were passaged several times in mammalian or mosquito cells only or alternatively in one and the other. In vivo experiments on mosquitoes compare the ability of these different strains to evolve and be transmitted. Furthermore, deep sequencing was performed in order to look for new minority variants in these different conditions for both viruses and strains. This work focuses on these two viruses’ genetic evolution to identify the mechanisms involved in transmission, and to predict the emergence of future variants.

Related team publications :
Stapleford, K. A. et al. Emergence and transmission of arbovirus evolutionary intermediates with epidemic potential. Cell Host Microbe 15, 706–716 (2014)
Stapleford, K. A. et al. Whole-Genome Sequencing Analysis from the Chikungunya Virus Caribbean Outbreak Reveals Novel Evolutionary Genomic Elements. PLoS Negl. Trop. Dis. 10, e0004402 (2016)
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Project Type : Medium
Status : Closed

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