Project #9635
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#9635 : Assembly of insect virus genome
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Name of Applicant : Karin Eiglmeier
Date of application : 21-09-2017
Unit : Genetic and Genomics of Insects Vectors
Location : Bâtiment Sergent, 3rd floor
Phone : 3718
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Project context and summary :

Mosquitoes of the Anopheles gambiae complex display strong preference for human blood- meals and are major malaria vectors in Africa. However, their interaction with viruses or role in arbovirus transmission during epidemics has been little examined, with the exception of O’nyong-nyong virus, closely related to Chikungunya virus. We have recently identified two novel insect RNA viruses, a Dicistrovirus and a Cypovirus, found in laboratory colonies of An. gambiae taxa using small-RNA deep sequencing. Wild-collected Anopheles from Senegal and Cambodia were positive for the Dicistrovirus and Cypovirus, displaying high sequence identity to the laboratory-derived virus. Thus, the Dicistrovirus (Anopheles C virus, AnCV) and Cypovirus (Anopheles Cypovirus, AnCPV) are components of the natural virome of at least some anopheline species. We purified the AnCPV virus by propagation on a insect cell line and we amplified and sequenced RNA extracted from the cellular supernatent. Our goal is to completely sequence the AnCPV, a dsRNA virus with an segmented genome.

Related team publications :
Carissimo G, Eiglmeier K, Reveillaud J, Holm I, Diallo M, Diallo D, et al. (2016) Identification and Characterization of Two Novel RNA Viruses from Anopheles gambiae Species Complex Mosquitoes. PLoS ONE 11(5): e0153881. doi:10.1371/journal. pone.0153881
Carissimo G, van den Beek M, Vernick KD, Antoniewski C (2017) Metavisitor, a Suite of Galaxy Tools for Simple and Rapid Detection and Discovery of Viruses in Deep Sequence Data. PLoS ONE 12(1): e0168397. doi:10.1371/journal. pone.0168397
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Project Type : Short
Status : In Progress

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