EVENT : C3BI Seminars
Conservation and co-evolution: from sequence analysis to protein-protein interactions
Speaker : Alessandra Carbone, from Laboratory of Computational and Quantitative Biology, CNRS Université Pierre et Marie Curie, Paris
Time : 02:00 pm
Starting Date : 21/01/2016
Location : Retrovirus room – LWOFF (22), Institut Pasteur, Paris
Conservation and co-evolution: from sequence analysis to protein-protein interactionsIn computational biology, a fundamental question is the extraction of evolutionary information from DNA sequences. Here, we consider protein sequences and structures. Given a family of protein sequences and the associated distance tree, we shall explain how a fine reading of the conservation and co-evolution signals between residues in sequences can be used to identify protein binding sites, mechanical and allosteric properties, protein-protein interactions. Based on this novel approach to coevolution analysis, we reconstructed the protein-protein interaction network of the Hepatitis C Virus at the residue resolution. For the first time, coevolution analysis of an entire virus was realised, based on a limited set of protein sequences with high sequence identity within genotypes. The identified coevolving residues constitute highly relevant predictions of protein-protein interactions for further experimental identification of HCV protein complexes. The method can be used for interaction predictions for other viral protein interaction networks.
Due to security policy in Institut Pasteur, please register before if you plan to come to this meeting