Hands-on High throughput Sequencing course in Bogota with the collaboration of Institut Pasteur and UniAndes

Hands-on course on High Throughput Sequencing data analysis: Genomics, Transcriptomics, Epigenomics

About

The University of Los Andes and the Hub of Bioinformatics and Biostatistics of the C3BI (Center of Bioinformatics Biostatistics and Integrative Biology) at Institut Pasteur will organize this Hands-on course aimed at students/researchers that have produced HTS data sets and would like to learn how to get the most out of it. This course will provide a general introduction to the bioinformatic and statistical aspects of HTS data analysis, alternating theory and practice. The objective is to present the general concepts such as file formats, mapping of sequences, as well as, the specific procedures for each data type: DNA-Seq, RNA-Seq, ChIP-Seq. An additional session will be devoted to the pathway and functional analysis for gene set issued from differential or other kind of analysis.
Knowledge of the basic Linux and R commands is highly recommended.
Please be aware that the workshop will be delivered both in Spanish and English.

Trainers

Programme

   
 
  • 08:00 – 8:30 Registration
  • 08:30 – 12:30
    • Presentation of the course
    • Introduction to HTS/NGS technologies (Illumina, PacBio, NanoPore, etc.) [T]
    • Basic stats/probability concepts: particularities of HTS/NGS sampling, underlying distributions, etc. [T]
  • 14:00 – 18:00
    • Quality analysis (error and bias of different technologies, quality metrics in sequencing, etc) [T/P]
    • Introduction to file formats (FASTA, FASTQ, SAM, BAM, BED, GFF …) [T/P]
 
  • 08:30 – 12:30
    • Alignment and mapping algorithms [T]
    • Mapping against a reference – Mapping for removal of contaminants [P]
  • 14:00 – 18:00
    • Assembly [T/P]
 
  • 08:30 – 12:30
    • Transcriptomics: Expression quantification, differential analysis, splice variant analysis [T/P]
  • 14:00 – 18:00
    • Epigenomics [T/P]: Quality control of IP enrichment, peak calling and introduction to differential analysis [T/P]
 
  • 08:30 – 12:30
    • Pathway analysis and functional analysis [T/P]
  • 14:00 – 18:00
    • Introduction to analysis in Galaxy
    • Visualization of data sets in the genome browser IGV
 

Registration

Registration is compulsory. Please proceed to the following link to complete your registration.

Location

Universidad de los Andes
Bogotá, Colombia
Carrera 1 #18A-12
https://uniandes.edu.co/en   Enregistrer